Phytochemical Profiling, Antioxidant And Antimicrobial Potentials Of Ethanol And Ethyl Acetate Extracts Of Kigelia Africana Stem Bark

Adepoju A. J., Esan A. O., Omodara N.B., Olawoore I. T., Adeoye A. O; Obisesan K. M., Haastrup B. S., Abegunrin A. I., Ayodele E.T., Ishola K.T.

Abstract


The research examines the phytochemical composition, antioxidant potential, and antibacterial properties of ethyl acetate (Kafr-EtOAc) and ethanolic (Kafr-EtOH) extracts from Kigelia africana (Kafr) that was harvested in Ogbomosho, Oyo state, Nigeria. Compared to Kafr-EtOAc, the Kafr-EtOH extract showed greater levels of total phenolic (33.67±0.17 GAE/mg/g), tannins (12.18±0.04 GAE/mg/g), flavonoids (27.89±0.05 QE/mg/g), alkaloids (7.22±0.09 %), and saponins (2.34±0.06 %). Important phenolic and sterol components were detected by GC-MS, such as 2, 3-dihydro-benzofuran (31.41 %), alpha-tocopherol (8.22 %), 2,6-hydroxycholesterol (18.68 %), and eugenol (5.13 %). The presence of distinctive functional groups was validated by FT-IR analysis. Strong DPPH scavenging capabilities were found in antioxidant studies; Kafr-EtOH exhibited the best findings (IC50 = 1.88 mg/mL). Furthermore, Kafr-EtOH demonstrated significant antibacterial effectiveness against the fungus Candida albicans as well as Gram-negative and Gram-positive bacteria. These results highlight the potential of ethanol extraction for pharmacological and nutraceutical uses by suggesting that it is a more effective method of separating bioactive components from Kafr.

Keywords


Kigelia africana; FT-IR; antioxidant activity; antimicrobials; GC-MS analysis

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References


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DOI: http://dx.doi.org/10.52155/ijpsat.v57.2.8124

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