Detection and Genotyping of Human Papillomavirus in Egyptian Head and Neck Cancer Patients

Doaa Salem, Sherif Bahaa, Mahmoud El Rouby, Abeer Bahnassy, yehia osman, Abdel-Rahman Zekri

Abstract


Background: The new classification of Head and Neck Squamous Cell Carcinoma (HNSCC) according to their association with human papillomavirus (HPV) infection into HPV positive tumors with good prognosis and HPV negative tumors with a poor prognosis has changed the treatment strategy of HNSCCs dramatically worldwide. However, data concerning HPV infection in HNSCCs patients is still limited and there are no studies regarding HPV genotypes in HNSCC among Egyptians. Therefore, the aim of the current study is to investigate the frequency of HPV and to detect the most prevalent HPV genotypes in Egyptian patients with oral cavity and oropharyngeal squamous cell carcinoma.

Methods: Ninety-two oral brushing samples (76 oral cavity and 16 oropharynx) were collected by an Orcellex Brush ® in CYTO-fast solution. The extracted DNA was subjected to two different molecular techniques; the e-BRID System™ for automatic detection and genotyping and the conventional PCR for detection of HPV DNA using consensus primers (MYO9/MYO11).

Results: Out of the 92 cases assessed, HPV DNA was found in three samples only (3/92; 3.3%) and the HPV66 genotype was the only genotype detected by the e-BRID System™.

Conclusion: Our findings may indicate that the HPV-associated HNSCC among Egyptian patients is very low and highlight the presence of HPV66 genotype in these malignancies. Other studies from different governorates in Egypt are still needed to determine the exact frequency of HPV infections in head and neck cancer.


Keywords


Human Papillomavirus; Flow-through hybridization; HPV66; Polymerase Chain Reaction; genotyping.

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References


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DOI: http://dx.doi.org/10.52155/ijpsat.v50.2.2996

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